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| Title: | EFFECTS OF AIR BUBBLES ON RABBIT BLOOD BRAIN BARRIER. |
| Authors: | Bolstad, G
Hjelde, A
Brubakk, AO |
| Keywords: | rabbit
animal
blood brain barrier
brain edema
air bubbles
experiment |
| Issue Date: | 1993 |
| Publisher: | Undersea and Hyperbaric Medical Society, Inc. |
| Abstract: | BACKGROUND: Under normal conditions cerebral, unlike peripheral blood vessels, are impermeable to circulating proteins due to a layer of endothelial cells. Several investigators have claimed that this Blood Brain Barrier (BBB) may be broken by circulating bubbles, resulting in brain tissue edema. We have earlier reported that we have been unable to verify this hypothesis by decompressing rats from 700 kPa of air. METHODS: In the present investigation three groups of six animals were infused with an isoosmotic solution of NaCl w/Macrodex and 1% Tween. The solution was saturated with air bubbles and infused at rates of 50-100 ml/hr, a total of 1.6, 3.3, or 6.6 ml in each group, respectively. Two groups of six animals each served as controls; one was infused by a degassed isoosmotic NaCl solution and one was sham operated. All animals were left for 30 mins after infusion before they were sacrificed, subsequently decapitated and their brains removed. Specific gravity of brain tissue samples was determined using a brombenzene/kerosene gradient, a decrease in specific gravity indicating local brain edema. RESULTS: Specific gravity of left hemisphere grey matter of bubble infused animals averaged 1.0409±0.001 as compared to 1.0421±0.0007 in sham the operated ones (p<0.005). Corresponding values for white matter were 1.0399±0.0016 and 1.0413±0.0010 (p<0.002). On the contralateral (right) side, white matter specific gravity averaged 1.0403±0.0011 in infused and 1.0414±0.0010 in sham operated animals (p<0.001), while the corresponding values in grey matter were 1.0413±0.0013 and 1.0422±0.0006 (p<0.0006). Infusion of degassed NaCl solution alone affected white, but not grey matter of both hemispheres. Specific gravity values for white matter of these animals averaged 1.0402±0.0007 (left, hemispheres p<0.001) and 1.0401±0.0008 (right hemisphere, p<0.001. CONCLUSION: This indicates that the infusion of NaCl, as such, at least partly may explain the formation of edema, in white matter, while this does not seem to be the case in grey matter. It is thus suggested that factors connected to infusion procedures may affect the BBB of rabbits. |
| Description: | Abstract of the Undersea and Hyperbaric Medical Society, Inc. Annual Scientific Meeting held July 7-10, 1993. World Trade and Convention Centre, Halifax, Nova Scotia, Canada. (http://www.uhms.org) |
| URI: | http://archive.rubicon-foundation.org/8216 |
| Appears in Collections: | UHMS Meeting Abstracts
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